Western blot analysis of extracts from HeLa cells untransfected (lane 1) or transfected with p73 DNA (lanes 2 and 3). Overexpression of p73 can be specifically blocked by co-transfection with p73 siRNA #6371 (lane 3). p73 was detected using p73 Antibody and p42 was detected using p42 MAPK Antibody #9108. The p73 Antibody confirms silencing of exogenous p73 expression and the p42 MAPK Antibody is used to control for loading and siRNA specificity.

Western blot analysis of extracts from HeLa cells untransfected (lane 1) or transfected with p73 DNA (lanes 2 and 3). Overexpression of p73 can be specifically blocked by co-transfection with p73 siRNA #6371 (lane 3). p73 was detected using p73 Antibody and p42 was detected using p42 MAPK Antibody #9108. The p73 Antibody confirms silencing of exogenous p73 expression and the p42 MAPK Antibody is used to control for loading and siRNA specificity.


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