The relationship between lysate protein concentration from untreated and PDGF treated NIH/3T3 cells and the absorbance at 450 nm using PathScan® Phospho-S6 Ribosomal Protein (Ser235/236) Sandwich ELISA Antibody Pair #7201 is shown. NIH/3T3 cells were starved overnight, treated with PDGF for 20 minutes at 37ºC and then lysed.