Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A,E), untreated human Bad transfected: sc-170552 (B,F), PMA treated human Bad transfected: sc-170552 (C,G) and PMA and lambda protein phosphatase treated human Bad transfected: sc-170552 (D,H) 293T whole cell lysates. Antibodies tested include p-Bad (C-10): sc-166932 (A,B,C) and Bad (H-168): sc-7869 (D,E,F).