Western blot analysis of caspase-1 (ICE) expression in HL-60 cells treated for zero (A,F), one (B,G), two (C,H), three (D,I) or four (E,J) hours with LPS. Antibodies tested include caspase-1 p10 (C-20): sc-515 (A-E) and caspase-1 (A-19): sc-622 (F-J).