Relationship between protein concentration of lysates from untreated and UV-treated 293 cells and immediate light generation with chemiluminescent substrate is shown. Cells (70-90% confluent) were treated with or without UV and lysed after incubation at 37ºC for 30 minutes. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

Relationship between protein concentration of lysates from untreated and UV-treated 293 cells and immediate light generation with chemiluminescent substrate is shown. Cells (70-90% confluent) were treated with or without UV and lysed after incubation at 37ºC for 30 minutes. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.


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