Western blot analysis of Nibrin phosphorylation in untreated (A, D), UV irradiated (B, E) and UV irradiated and lambda protein phosphatase treated (C, F) HeLa whole cell lysates. Antibodies tested include p-Nibrin (Ser 343)-R: sc-12936-R (A, B, C) and Nibrin (1D7): sc-56166 (D, E, F).