Western blot analysis of Rb phosphorylation in non-transfected: sc-117752 (A,D), untreated human Rb transfected: sc-114014 (B,E) and lambda protein phosphatase treated human Rb transfected: sc-114014 (C,F) 293T whole cell lysates. Antibodies tested include p-Rb (83.T821/826): sc-135777 (A,B,C) and Rb (M-153): sc-7905 (D,E,F).