ab9050 staining Histone H3 (tri methyl K36) in MCF-7 cells. The cells were incubated with 15µM Methylstat (ab144566) for 24 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9050 at 0.1µg/ml and ab195889 Mouse monoclonal [DM1A] to alpha Tubulin - Alexa Fluor® 594 at 2µg/ml (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 1/1000 dilution (shown in green). Nuclear DNA was labelled in blue with DAPI. Negative controls: 1– anti-rabbit secondary only (solvent only); 2 – anti-rabbit secondary only (Treated). The secondary antibody control demonstrates that the labelling observed is due only to the binding of the secondary antibody to the primary antibody

ab9050 staining Histone H3 (tri methyl K36) in MCF-7 cells. The cells were incubated with 15µM Methylstat (ab144566) for 24 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9050 at 0.1µg/ml and ab195889 Mouse monoclonal [DM1A] to alpha Tubulin - Alexa Fluor® 594 at 2µg/ml (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 1/1000 dilution (shown in green). Nuclear DNA was labelled in blue with DAPI. Negative controls: 1– anti-rabbit secondary only (solvent only); 2 – anti-rabbit secondary only (Treated). The secondary antibody control demonstrates that the labelling observed is due only to the binding of the secondary antibody to the primary antibody


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