Western blot analysis of tuberin phosphorylation in untreated (A,D), PMA/Calyculin A treated (B,E) and PMA/Calyculin A and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-tuberin (325.Ser 1798): sc-293149 (A,B,C) and tuberin (B-5): sc-271314 (D,E,F).