Western blot analysis of Cdc2 p34 phosphorylation in untreated (A,F), Hydroxyurea treated (B,G), nocodazole treated (C,H), Hydroxyurea and lambda protein phosphatase treated (D,I) and nocodazole and lambda protein phosphatase treated (E,J) HeLa whole cell lysates. Antibodies tested include p-Cdc2 p34 (Tyr 15)-R: sc-7989-R (A-E) and p-Cdc2 p34 (PSTAIRE): sc-53 (F-J).

Western blot analysis of Cdc2 p34 phosphorylation in untreated (A,F), Hydroxyurea treated (B,G), nocodazole treated (C,H), Hydroxyurea and lambda protein phosphatase treated (D,I) and nocodazole and lambda protein phosphatase treated (E,J) HeLa whole cell lysates. Antibodies tested include p-Cdc2 p34 (Tyr 15)-R: sc-7989-R (A-E) and p-Cdc2 p34 (PSTAIRE): sc-53 (F-J).


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