Western blot analysis of Cdc2 p34 phosphorylation in untreated (A,D), nocodazole treated (B,E) and nocodazole and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-Cdc2 p34 (Thr 161)-R: sc-12341-R (A,B,C) and p-Cdc2 p34 (PSTAIRE): sc-53 (D,E,F).