Western blot analysis of Akt1 phosphorylation in A-431 cells treated with EGF. Blots were probed with Akt1 (C-20): sc-1618 (A), p-Akt1/2/3 (Ser 473): sc-7985-R preincubated with cognate unphosphorylated peptide (B) and p-Akt1/2/3 (Ser 473): sc-7985-R preincubated with cognate phosphorylated peptide (C).