Western blot analysis of p21 phosphorylation in untreated (A,D), human recombinant Akt1 treated (B,E) and human recombinant Akt1 and lambda protein phosphatase treated (C,F) human recombinant p21 fusion proteins. Antibodies tested include p-p21 (Thr 145)-R: sc-20220-R (A,B,C) and p21 (C-19): sc-397 (D,E,F).