Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Bcl-2 siRNA I (+) or SignalSilence® Bcl-2 siRNA II #6516 (+), using Bcl-2 (50E3) Rabbit mAb and α-Tubulin (11H10) Rabbit mAb #2125. Bcl-2 (50E3) Rabbit mAb #2870 confirms silencing of Bcl-2 expression, while the α-tubulin (11H10) rabbit mAb is used to control for loading and specificity of Bcl-2 siRNA.