Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human brachyury (hBrachyury-Myc/DDK; +), using Brachyury (D5Y9I) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).