Western blot analysis of extracts from HT-29 cells, untreated (-) or pre-treated with Z-VAD (20 μM, 30 min) followed by overnight treatment with Cycloheximide #2112 (CHX, 10 μg/ml) and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml; +), using Phospho-MLKL (Thr357/Ser358) Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from HT-29 cells, untreated (-) or pre-treated with Z-VAD (20 μM, 30 min) followed by overnight treatment with Cycloheximide #2112 (CHX, 10 μg/ml) and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml; +), using Phospho-MLKL (Thr357/Ser358) Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


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