Western blot analysis of extracts from wild-type and PKCθ (-/-) mouse splenocytes using PKCθ (P632) Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Extracts from wild-type and PKCθ (-/-) mouse splenocytes were kindly provided by Dr. Morgan Huse (Memorial Sloan-Kettering Cancer Center).