1D SDS-PAGE of ab88382 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab88382 Lane 2: ab88382 treated with PNGase F to remove potential N-linked glycans Lane 3: ab88382 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Drop in MWt after treatment with PNGase F indicates the presence of N-linked glycans. Subsequent small drop in MWt after treatment with glycosidase cocktail suggests possible presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
A sample of ab88382 without carrier protein was reduced and alkylated. 40 µg protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot trains (Deep Purple™ stained) indicates the presence of multiple isoforms of GM-CSF.
Densitometry of protein isoforms visualised by 2-DE. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates the theoretical MWt and pI of the protein.