Active human htrA4 full length protein
| Name | Active human htrA4 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab134444 |
| Category | Protein |
| Prices | $840.00 |
| Sizes | 50 µl |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Insect cells |
| Tag/Conjugation | His tag C-Terminus |
| Purity | > 70 % by SDS-PAGE. |
| Bioactivity | Proteolytic activity of ab134444 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 10 µg/ml htrA4 within 18 hours at 37°C. |
| SwissProt/Accession | P83105 |
| Gene | HTRA4 |
| Residue | 1 to 476 |
| Sequence | MIRPQLRTAGLGRCLLPGLLLLLVPVLWAGAEKLHTQPSCPAVCQPTRCP ALPTCALGTTPVFDLCRCCRVCPAAEREVCGGAQGQPCAPGLQCLQPLRP GFPSTCGCPTLGGAVCGSDRRTYPSMCALRAENRAARRLGKVPAVPVQWG NCGDTGTRSAGPLRRNYNFIAAVVEKVAPSVVHVQLWGRLLHGSRLVPVY SGSGFIVSEDGLIITNAHVVRNQQWIEVVLQNGARYEAVVKDIDLKLDLA VIKIESNAELPVLMLGRSSDLRAGEFVVALGSPFSLQNTATAGIVSTKQR GGKELGMKDSDMDYVQIDATINYGNSGGPLVNLDGDVIGVNSLRVTDGIS FAIPSDRVRQFLAEYHEHQMKGKAFSNKKYLGLQMLSLTVPLSEELKMHY PDFPDVSSGVYVCKVVEGTAAQSSGLRDHDVIVNINGKPITTTTDVVKAL DSDSLSMAVLRGKDNLLLTVIPETINHHHHHH |
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Product images
SDS-PAGE analysis of ab134444 (1.5µg).
Hydrolysis of ß-Casein by ab134444. ß-Casein (0.19 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM CaCl 2 with or without HtrA4 (43.7 µg/ml). After indicated time intervals 4 µl aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE. Lane 1: 0 hours Lane 2: 2 hours Lane 3: 6 hours Lane 4: 18 hours