Active human IDH2 full length protein
| Name | Active human IDH2 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198092 |
| Category | Protein |
| Prices | $359.00 |
| Sizes | 50 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | DDDDK tag C-Terminus |
| Purity | >= 59 % by SDS-PAGE. |
| Bioactivity | Specific Activity: 14,200 pmol/min/µg. Assay Conditions: The reaction mixture contained 50 mM Tris, pH 7.4, 5 mM MgCl 2 , 200 µM DL-isocitrate, 200 µM NADP+, and various amount of ab198092 in a volume of 200 µL. Reactions were initiated with the addition of ab198092, and the increase in NADPH absorbance at 340 nm was continuously monitored for 7 min. Molar extinction coefficient of NADPH is 6,220 M -1 cm -1 . . |
| SwissProt/Accession | P48735 |
| Gene | IDH2 |
| Residue | 1 to 452 |
| Sequence | MAGYLRVVRSLCRASGSRPAWAPAALTAPTSQEQPRRHYADKRIKVAKPV VEMDGDEMTRIIWQFIKEKLILPHVDIQLKYFDLGLPNRDQTDDQVTIDS ALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFRE PIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKD GSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMST KNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKS SGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGT VTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEK VCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALG RQDYKDDDDK |
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