Human FOXP3 peptide
| Name | Human FOXP3 peptide |
|---|---|
| Supplier | Abcam |
| Catalog | ab14151 |
| Category | Peptide |
| Applications | B/N |
| For Antibody | Anti-FOXP3 antibody |
| Species Reactivities | Human |
| Nature | Synthetic |
| SwissProt/Accession | Q9BZS1 |
| Gene | FOXP3 |
| Sequence | Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. |
| Supplier Page | Shop |
Product images
Lanes 1 - 6 : Anti-FOXP3 antibody ( ab10563 ) at 2 µg/ml Lane 7 : Lane 1 : 20µg HEK 293 lysate Lane 2 : 20µg HEK 293 lysate over expressing human FOXP3 Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3 Lane 4 : 20µg HEK 293 lysate with Human FOXP3 peptide (ab14151) at 1 µg/ml Lane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with Human FOXP3 peptide (ab14151) at 1 µg/ml Lane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with Human FOXP3 peptide (ab14151) at 1 µg/ml Lane 7 : 20µg HEK 293 lysate Secondary Goat Anti-Rabbit IgG H&L (HRP) ( ab6721 ) at 0.2 µg/ml Performed under reducing conditions. Predicted band size : 47.2 kDa Observed band size : 47 kDa ( why is the actual band size different from the predicted? ) Exposure time : 30 seconds This antibody recognises a single clean band in a HEK293 cell lysate that overexpresses human FOXP3. The band is not seen in untransfected cells and is blocked by the immunising peptide.
Product References
Regulatory T cells in the skin lesions and blood of patients with systemic - Regulatory T cells in the skin lesions and blood of patients with systemic
Antiga E, Quaglino P, Bellandi S, Volpi W, Del Bianco E, Comessatti A, Osella-Abate S, De Simone C, Marzano A, Bernengo MG, Fabbri P, Caproni M. Br J Dermatol. 2010 May;162(5):1056-63.
.
FoxP3-expressing T regulatory cells in atopic dermatitis lesions. - FoxP3-expressing T regulatory cells in atopic dermatitis lesions.
Caproni M, Antiga E, Torchia D, Volpi W, Barletta E, Gitti G, De Campora E, Fabbri P. Allergy Asthma Proc. 2007 Sep-Oct;28(5):525-8.
Absence of T-regulatory cell expression and function in atopic dermatitis skin. - Absence of T-regulatory cell expression and function in atopic dermatitis skin.
Verhagen J, Akdis M, Traidl-Hoffmann C, Schmid-Grendelmeier P, Hijnen D, Knol EF, Behrendt H, Blaser K, Akdis CA. J Allergy Clin Immunol. 2006 Jan;117(1):176-83.