Human FOXP3 peptide

• Supplier: Abcam
• Catalog: ab16809
• Category: Peptide
• Applications: B/N
• For Antibody: Anti-FOXP3 antibody [mAbcam 22510] (HRP)
• Nature: Synthetic
• SwissProt/Accession: Q9BZS1
• Gene: FOXP3
• Sequence: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain.

Product images

All lanes : Anti-FOXP3 antibody ( ab10901 ) at 0.4 µg/ml Lane 1 : 20µg HEK 293 lysate Lane 2 : 20µg HEK 293 lysate over expressing human FOXP3 Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3 Lane 4 : 20µg HEK 293 lysate with Human FOXP3 peptide (ab16809) at 1 µg/ml Lane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml Lane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml Secondary Lanes 4 - 6 : Goat Anti-Rabbit IgG H&L (HRP) ( ab6721 ) at 0.2 µg/ml Performed under reducing conditions. Predicted band size : 47 kDa Exposure time : 1 minute A no primary control lane showed no bands.

All lanes : Anti-FOXP3 antibody [mAbcam 450] ( ab450 ) at 1 µg/ml Lane 1 : HEK293 cells overexpressing human FOXP3 Lane 2 : HEK293 cells overexpressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Rabbit Anti-Mouse IgG H&L (HRP) ( ab6728 ) at 1/5000 dilution Performed under reducing conditions. Predicted band size : 48 kDa Observed band size : 50 kDa ( why is the actual band size different from the predicted? ) Exposure time : 45 seconds

Product References

Characterization of human invariant natural killer T cells expressing FoxP3. - Characterization of human invariant natural killer T cells expressing FoxP3.

Engelmann P, Farkas K, Kis J, Richman G, Zhang Z, Liew CW, Borowiec M, Niewczas MA, Jalahej H, Orban T. Int Immunol. 2011 Aug;23(8):473-84.