1D SDS-PAGE of ab85600 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab85600 Lane 2: ab85600 treated with PNGase F to remove potential N-linked glycans Lane 3: ab85600 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.