All lanes : Anti-gamma Tubulin antibody - Centrosome Marker ( ab16504 ) at 1 µg/ml Lane 1 : HeLa Whole Cell Lysate Lane 2 : HeLa Nuclear Cell Lysate Lane 3 : A431 Whole Cell Lysate Lane 4 : HeLa Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml Lane 5 : HeLa Nuclear Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml Lane 6 : A431 Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution Performed under reducing conditions. Predicted band size : 48 kDa Observed band size : 48 kDa