IL4RA protein (Fc Chimera)
| Name | IL4RA protein (Fc Chimera) |
|---|---|
| Supplier | Abcam |
| Catalog | ab83756 |
| Category | Protein |
| Prices | $315.00 |
| Sizes | 25 µg |
| Applications | SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | The ED 50 of IL4R - Fc Chimera is typically 45-60 ng/ml as measured by its ability to neutralize IL-4 mediated proliferation of the human growth factor dependent TF-1 cell line. |
| SwissProt/Accession | P24394 |
| Gene | IL4R |
| Residue | 1 to 232 |
| Sequence | Theoretical Sequence: MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQL VFLLSEAHTCIPEN NGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGN L TVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTY LEP SLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQH G SSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR TPE VTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTV LH QDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTK NQV SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVD KSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
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Product images
Lane 1: MW markers; Lane 2: IL4R - Fc Chimera; Lane 3: IL4R - Fc Chimera treated with PNGase F to remove potential N-linked glycans; Lane 4: IL4R - Fc Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins. The triangle indicates theoretical pI and MW of the protein.