Lanes 1 & 3 : Anti-Histone H2A (di methyl K95) antibody ( ab4633 ) at 1/500 dilution Lanes 2 & 4 : Anti-Histone H2A (di methyl K95) antibody ( ab4633 ) at 1/1000 dilution Lane 1 : 1ug of calf thymus Histone prep Lane 2 : 1ug of calf thymus Histone prep Lane 3 : 1ug of calf thymus Histone prep with Human Histone H2A (di methyl K95) peptide (ab10127) at 2 µg Lane 4 : 1ug of calf thymus Histone prep with Human Histone H2A (di methyl K95) peptide (ab10127) at 2 µg Secondary Lanes 1 - 3 : Goat Anti-Rabbit IgG H&L (HRP) ( ab6721 ) at 2000 Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) ( ab6721 ) at 1/2000 dilution