All lanes : Anti-Histone H2B (acetyl K11) antibody ( ab40975 ) at 1 µg/ml Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate Lane 2 : Histone H2B Human recombinant Lane 3 : Histone H3.1 Human recombinant Lane 4 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H2B (acetyl K11) peptide (ab41888) at 1 µg/ml Lane 5 : Histone H2B Human recombinant with Human Histone H2B (acetyl K11) peptide (ab41888) at 1 µg/ml Lane 6 : Histone H3.1 Human recombinant with Human Histone H2B (acetyl K11) peptide (ab41888) at 1 µg/ml Lane 7 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H2B peptide ( ab35791 ) at 1 µg/ml Lane 8 : Histone H2B Human recombinant with Human Histone H2B peptide ( ab35791 ) at 1 µg/ml Lane 9 : Histone H3.1 Human recombinant with Human Histone H2B peptide ( ab35791 ) at 1 µg/ml Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing conditions. Predi