All lanes : Anti-Histone H3 (acetyl K23) antibody ( ab47813 ) at 1 µg/ml Lane 1 : HeLa Histone Preparation Nuclear Lysate - Butyrated Lane 2 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 peptide ( ab17163 ) at 0.5 µg/ml Lane 3 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Histone H3 peptide - acetyl K36 at 0.5 µg/ml Lane 4 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K9) peptide ( ab16635 ) at 0.5 µg/ml Lane 5 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Histone H3 peptide - acetyl K14 at 0.5 µg/ml Lane 6 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K18) peptide ( ab24003 ) at 0.5 µg/ml Lane 7 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K27) peptide ( ab24404 ) at 0.5 µg/ml Lane 8 : HeLa Histone Preparation Nuclear Lysate - Butyrated with Human Histone H3 (acetyl K23) peptide (ab48359) at 0.5 µg/ml Lysates/protein