ab31388 specifically recognises mono-methyl K27 (lane 1). ab31388 is successfully blocked using the immunizing peptide (lane 3 ab1780), but not the corresponding unmodified peptides (lane 2 ab2903 , lane 6 ab2623 ) nor di-methyl K27 (lane 4 ab1781 ), tri-methyl K27 (lane 5 ab1782 ), mono-methyl K9 (lane 7 ab1771 ), mono-methyl K4 (lane 8 ab1340 ) peptides. The bands at 50 kDa are attributed to non-specific binding of the antibody.
ab31388 specifically recognises mono-methyl K27 (lane 1). ab31388 is successfully blocked using the immunizing peptide (lane 3 ab1780), but not the corresponding unmodified peptides (lane 2 ab2903 , lane 6 ab2623 ) nor di-methyl K27 (lane 4 ab1781 ), tri-methyl K27 (lane 5 ab1782 ), mono-methyl K9 (lane 7 ab1771 ), mono-methyl K4 (lane 8 ab1340 ) peptides. The bands at 50 kDa are attributed to non-specific binding of the antibody.
ab31388 specifically recognises mono-methyl K27 (lane 1). ab31388 is successfully blocked using the immunizing peptide (lane 3 ab1780), but not the corresponding unmodified peptides (lane 2 ab2903 , lane 6 ab2623 ) nor di-methyl K27 (lane 4 ab1781 ), tri-methyl K27 (lane 5 ab1782 ), mono-methyl K9 (lane 7 ab1771 ), mono-methyl K4 (lane 8 ab1340 ) peptides. The bands at 50 kDa are attributed to non-specific binding of the antibody.
ab31399 specifically recognises mono-methyl K27 (lane 1). ab31399 is successfully blocked using the immunizing peptide (lane 3 ab1780), but not the corresponding unmodified peptides (lane 2 ab2903 , lane 6 ab2623 ) nor di-methyl K27 (lane 4 ab1781 ), tri-methyl K27 (lane 5 ab1782 ), mono-methyl K9 (lane 7 ab1771 ), mono-methyl K4 (lane 8 ab1340 ) peptides.
ab31399 specifically recognises mono-methyl K27 (lane 1). ab31399 is successfully blocked using the immunizing peptide (lane 3 ab1780), but not the corresponding unmodified peptides (lane 2 ab2903 , lane 6 ab2623 ) nor di-methyl K27 (lane 4 ab1781 ), tri-methyl K27 (lane 5 ab1782 ), mono-methyl K9 (lane 7 ab1771 ), mono-methyl K4 (lane 8 ab1340 ) peptides.
ab31399 specifically recognises mono-methyl K27 (lane 1). ab31399 is successfully blocked using the immunizing peptide (lane 3 ab1780), but not the corresponding unmodified peptides (lane 2 ab2903 , lane 6 ab2623 ) nor di-methyl K27 (lane 4 ab1781 ), tri-methyl K27 (lane 5 ab1782 ), mono-methyl K9 (lane 7 ab1771 ), mono-methyl K4 (lane 8 ab1340 ) peptides.