All lanes : Anti-Histone H4 (Biotinylated K5) antibody ( ab17343 ) at 1 µg/ml Lane 1 : HeLa nuclear extract Lane 2 : HeLa nuclear extract with Human Histone H4 (biotinylated K5) peptide (ab17632) at 1 µg/ml Lane 3 : HeLa nuclear extract with Human Histone H4 peptide ( ab15825 ) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab7090 ) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 11 kDa Observed band size : 11 kDa See Abreview