| Name |
ProDots Recombinant Human FGF-3 Protein
|
| Supplier |
R&D Systems
|
| Catalog |
PRD1206
|
| Category |
Protein
|
| Prices |
$109.00, $499.00, $1,399.00
|
| Sizes |
5 µg, 50 µg, 250 µg
|
| Species Reactivities |
Human
|
| Nature |
Recombinant
|
| Source |
E. coli -derived Asp28 - Arg212, with an N-terminal Met
|
| Purity |
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
|
| Bioactivity |
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al . (1988) Cancer Res. 48 :4266; Thomas, K. et al . (1987) Methods Enzymol. 147 :120. The ED50 for this effect is 0.02-0.1 μg/mL in the presence of 1 μg/mL of heparin.
|
| Endotoxin |
<0.01 EU per 1 μg of the protein by the LAL method.
|
| SwissProt/Accession |
NP_005238
|
| Gene |
FGF3
|
| Sequence |
Met
|
| Supplier Page |
Shop
|
1 μg/lane of ProDots ® Recombinant Human FGF-3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 21 kDa.
ProDots ® Recombinant Human FGF-3 stimulated cell proliferation of the NR6R-3T3 mouse fibroblast cell line. The ED 50 for this effect is typically 0.02-0.1 μg/mL.