Active human KDM3A / JHDM2A full length protein
| Name | Active human KDM3A / JHDM2A full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab196411 |
| Category | Protein |
| Prices | $1,265.00 |
| Sizes | 20 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | DDDDK tag N-Terminus |
| Purity | >= 27 % by SDS-PAGE. ab196411 is greater or equal to 27% pure as assessed by SDS-PAGE. |
| Bioactivity | 0.3 pmole/min/μg: 10µl reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab196411 (20 – 100 ng) added to the wells. Add antibody against mono-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. |
| SwissProt/Accession | Q9Y4C1 |
| Gene | KDM3A |
| Residue | 2 to 1321 |
| Sequence | VLTLGESWPVLVGRRFLSLSAADGSDGSHDSWDVERVAEWPWLSGTIRAV SHTDVTKKDLKVCVEFDGESWRKRRWIEVYSLLRRAFLVEHNLVLAERKS PEISERIVQWPAITYKPLLDKAGLGSITSVRFLGDQQRVFLSKDLLKPIQ DVNSLRLSLTDNQIVSKEFQALIVKHLDESHLLKGDKNLVGSEVKIYSLD PSTQWFSATVINGNPASKTLQVNCEEIPALKIVDPSLIHVEVVHDNLVTC GNSARIGAVKRKSSENNGTLVSKQAKSCSEASPSMCPVQSVPTTVFKEIL LGCTAATPPSKDPRQQSTPQAANSPPNLGAKIPQGCHKQSLPEEISSCLN TKSEALRTKPDVCKAGLLSKSSQIGTGDLKILTEPKGSCTQPKTNTDQEN RLESVPQALTGLPKECLPTKASSKAELEIANPPELQKHLEHAPSPSDVSN APEVKAGVNSDSPNNCSGKKVEPSALACRSQNLKESSVKVDNESCCSRSN NKIQNAPSRKSVLTDPAKLKKLQQSGEAFVQDDSCVNIVAQLPKCRECRL DSLRKDKEQQKDSPVFCRFFHFRRLQFNKHGVLRVEGFLTPNKYDNEAIG LWLPLTKNVVGIDLDTAKYILANIGDHFCQMVISEKEAMSTIEPHRQVAW KRAVKGVREMCDVCDTTIFNLHWVCPRCGFGVCVDCYRMKRKNCQQGAAY KTFSWLKCVKSQIHEPENLMPTQIIPGKALYDVGDIVHSVRAKWGIKANC PCSNRQFKLFSKPASKEDLKQTSLAGEKPTLGAVLQQNPSVLEPAAVGGE AASKPAGSMKPACPASTSPLNWLADLTSGNVNKENKEKQPTMPILKNEIK CLPPLPPLSKSSTVLHTFNSTILTPVSNNNSGFLRNLLNSSTGKTENGLK NTPKILDDIFASLVQNKTTSDLSKRPQGLTIKPSILGFDTPHYWLCDNRL LCLQDPNNKSNWNVFRECWKQGQPVMVSGVH |
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