Active human KDM4D / JMJD2D protein fragment
| Name | Active human KDM4D / JMJD2D protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab196398 |
| Category | Protein |
| Prices | $858.00 |
| Sizes | 100 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | >90% by SDS-PAGE. |
| Bioactivity | Specific Activity: 0.017 pmole/min/μg Assay Conditions: 10µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab196398 (100 – 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection. |
| SwissProt/Accession | Q6B0I6 |
| Gene | KDM4D |
| Residue | 2 to 354 |
| Sequence | ETMKSKANCAQNPNCNIMIFHPTKEEFNDFDKYIAYMESQGAHRAGLAKI IPPKEWKARETYDNISEILIATPLQQVASGRAGVFTQYHKKKKAMTVGEY RHLANSKKYQTPPHQNFEDLERKYWKNRIYNSPIYGADISGSLFDENTKQ WNLGHLGTIQDLLEKECGVVIEGVNTPYLYFGMWKTTFAWHTEDMDLYSI NYLHLGEPKTWYVVPPEHGQRLERLARELFPGSSRGCGAFLRHKVALISP TVLKENGIPFNRITQEAGEFMVTFPYGYHAGFNHGFNCAEAINFATPRWI DYGKMASQCSCGEARVTFSMDAFVRILQPERYDLWKRGQDRAVVDHMEPR VPA |
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