Active human EHMT2/ G9A protein fragment
| Name | Active human EHMT2/ G9A protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab198156 |
| Category | Protein |
| Prices | $1,250.00 |
| Sizes | 20 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | >= 50 % by SDS-PAGE. |
| Bioactivity | Specific Activity: ≥70 pmol/min/µg Assay conditions: 50 μl reaction mix (50 mM TRIS pH 8.8, 5mM MgCl 2 , 4mM DTT, 0.05% Tween-20, 40 μM S-adenosylmethionine, and ab198156 (0.05 – 0.5 ng) add to the wells coated with the substrate on a Neutravidin black plate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP- labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. |
| SwissProt/Accession | Q96KQ7 |
| Gene | EHMT2 |
| Residue | 785 to 1210 |
| Sequence | GWTPIIWAAEHKHIEVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIA EVLLNARCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEG DTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVP IPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSN CLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQS GIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDS YLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFP RIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIAL EQSRLARLDPHPELLPELGS LPPVNT |
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