Active human Lactate Dehydrogenase B full length protein

Name Active human Lactate Dehydrogenase B full length protein
Supplier Abcam
Catalog ab96765
Category Protein
Prices $200.00, $706.00
Sizes 100 µg, 500 µg
Applications MS SDS-PAGE
Species Reactivities Human
Nature Recombinant
Source E. coli
Tag/Conjugation His tag N-Terminus
Purity > 95 % by SDS-PAGE. ab96765 is purified by using conventional chromatography techniques.
Bioactivity Specific activity is > 6 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C. Activity Assay Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH7.5 at 37°C, and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer Add 50ul of recombinant LDHB protein in various concentrations (0.1ug, 0.5ug) in assay buffer. Mix by inversion and record the increase at A340nm for 5 minutes.
SwissProt/Accession P07195
Gene LDHB
Residue 1 to 334
Sequence MGSSHHHHHH SSGLVPRGSH MATLKEKLIAPVAEEEATVP NNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGS LFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKF IIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSAR FRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMG TDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSR IHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKS ADTLWDIQKDLKDL
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