Recombinant BRPF1 (629-742) Protein SDS-PAGE Gel. BRPF1 (629-742) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.
HTRF assay for BRPF1 (629-742) protein activity. 3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-tag in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature.
HTRF assay for BRPF1 (629-742) protein activity. 3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-tag in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature.