Recombinant Mononucleosomes (H2A.Bbd) - biotinylated

• Supplier: ActiveMotif
• Catalog: 31556
• Prices: $415.00
• Sizes: 20 µg
• Species Reactivities: Human
• Nature: Recombinant
• Source: E. coli
• Gene: H2AFB2
• Description: Recombinant Mononucleosomes (H2A.Bbd) - biotinylated, consist of 167 bp of 601 DNA with a 5’ biotin tag and two molecules each of histones H2A.Bbd that includes amino acids 1-115 (end) (accession number NP_001017991.1), H2B that includes amino acids 1-126 (end) (accession number NP_003509.1), H3 that includes amino acids 1-136 (end) (accession number NP_003520.1), and H4 that includes amino acids 1-103 (end) (accession number NP_003539.1)

Product images

Streptavidin Pull-down for Recombinant Mononucleosomes (H2A. Bbd) - biotinylated Mononucleosomes (H2A. Bbd) were pulled down by streptavidin beads. Input mononucleosomes (Lane 1) and the mononucleosomes pulled down by streptavidin (Lane 2) were run on a 12. 5% SDS-PAGE gel and stained with Coomassie Blue. The SDS-PAGE gel result shows that more than 80% biotinylated nucleosomes (H2A. Bbd) are pulled down by streptavidin beads. * indicates streptavidin.

Recombinant Mononucleosomes (H2A. Bbd) - biotinylated DNA gel Biotinylated Mononucleosomes (H2A. Bbd) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: Intact mononucleosomes. Lane 2: 601 DNA which was used for assembly of mononucleosomes. Intact mononucleosomes migrate much higher than free DNA. The agarose gel result shows that almost all of 601 DNA wrap histone octamers to form mononucleosomes.

Western Blot analysis for Mononucleosomes (H2A. Bbd) - biotinylated 2 µg Recombinant Mononucleosomes (H2A. Bbd) were incubated with DOT1L (Cat# 31474) in reaction buffer for 3 h at room temperature. Western Blot was used for detecting the generation of reaction products (H3K79me1, Cat# 39921). DOT1L only and mononnucleosomes only were used as negative control. The Western Blot result shows that mononucleosomes are more suitable substrate for DOT1L than histone octamers.