SDS-PAGE/Western blot analysis of ab129131. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue (lanes 1-3). Lane 1: Molecular weight marker (kDa) Lane 2: 0.05 ug ab129131 Lane 3: 0.3 ug ab129131 ab129131 was electrophoresed under reducing conditions on a 15% SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody to Human Growth Hormone. Lane 4: immune-detection with specific anti-Human Growth Hormone 0.05 ug Lane 5: immune-detection with specific anti-Human Growth Hormone 0.3 ug