Active human LDHA full length protein
| Name | Active human LDHA full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab93699 |
| Category | Protein |
| Prices | $200.00, $706.00 |
| Sizes | 100 µg, 500 µg |
| Applications | SDS-PAGE WB |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Purity | > 95 % by SDS-PAGE. ab93699 is purified by using conventional chromatography techniques. |
| Bioactivity | Specific activity is > 20 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C. Activity Assay Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug, 0.5ug) in assay buffer. Mix by inversion and record the increase at A340nm for 5 minutes. |
| SwissProt/Accession | P00338 |
| Gene | LDHA |
| Sequence | MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF |
| Supplier Page | Shop |
Product images
15% SDS-PAGE showing ab93699 at approximately 38.8kDa (3µg).