Densitometry of protein isoforms visualised by 2-DE. The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83919 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab83919 Lane 2: ab83919 treated with PNGase F to remove potential N-linked glycans Lane 3: ab83919 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Deep Purple™.