1D SDS-PAGE of ab83828 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab83828 Lane 2: ab83828 treated with PNGase F to remove potential N-linked glycans Lane 3: ab83828 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 μg protein loaded per lane; Deep Purple™ stained.
A sample of ab83828 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained.
Densitometry of protein isoforms visualised by 2-DE. The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.