Densitometry of protein isoforms visualised by 2-DE. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83827 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab83827 Lane 2: ab83827 treated with PNGase F to remove potential N-linked glycans 10 μg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans.