Active human MLL + ASH2L + RBBP5 + WDR5 full length protein
| Name | Active human MLL + ASH2L + RBBP5 + WDR5 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab135021 |
| Category | Protein |
| Prices | $820.00 |
| Sizes | 50 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Purity | >= 80 % by SDS-PAGE. |
| Bioactivity | 50 µl reaction mix (50 mM Tris, pH 8.8, 5 mM MgCl 2 , 4 M DTT, 20 µM Sadenosylmethionine, and 5-50 ng MLL complex) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then, incubate 30 minutes with the secondary HRP labeled antibody. Finally, add HRP chemiluminescent substrate and read luminescence. |
| Gene | KMT2A |
| Sequence | MLL FRFHKPEEANEPPLNPHGSARAEVHLRKSAFDMFNFLASKHRQPPEYN PNDEEEEEVQLKSARRATSMDLPMPMRFRHLKKTSKEAVGVYRSPIHGRG LFCKRNIDAGEMVIEYAGNVIRSIQTDKREKYYDSKGIGCYMFRIDDSEV VDATMHGNAARFINHSCEPNCYSRVINIDGQKHIVIFAMRKIYRGEELTY DYKFPIEDASNKLPCNCGAKKCRKFLN WDR5 ATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSV KFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNL LVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDE SVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTA SGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKT YTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHT DVVISTACHPTENIIASAALENDKTIKLWKSDC ASH2L Isoform 2 DTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFMTNY SFHCNVCHHSGNTYFLRKQANLKEMCLSALANLTWQSRTQDEHPKTMFSK DKDIIPFIDKYWECMTTRQRPGKMTWPNNIVKTMSKERDVFLVKEHPDPG SKDPEEDYPKFGLLDQDLSNIGPAYDNQKQSSAVSTSGNLNGGIAAGSSG KGRGAKRKQQDGGTTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRY ILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLK ISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVDEMPPDTAARLGWSQP LGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLP |
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