All lanes : Anti-LAR antibody ( ab10557 ) at 2 µg/ml Lane 1 : Liver lysate (18 µg/lane) Lane 2 : Liver lysate (18 µg/lane) with Human LAR peptide (ab22637) at 1 µg/ml Performed under reducing conditions. Predicted band size : 211 kDa Observed band size : 150 kDa ( why is the actual band size different from the predicted? ) Additional bands at : 50 kDa (possible isoform),70 kDa (possible isoform). ab10557 detects a band of about 150 kDa, which corresponds to the reported mobility of the whole protein and two bands of 60 kDa and 50 kDa, which may correspond to isoforms or processed forms of LAR. ab22367 blocking/immunizing peptide for LAR antibody ab10557 , fully quenches all bands in WB.