| Name | NAD-dependent malic enzyme, Recombinant Protein |
|---|---|
| Supplier | MyBioSource |
| Catalog | MBS969128 |
| Category | Protein |
| Prices | $115.00, $205.00, $395.00, $675.00 |
| Sizes | 5 µg, 25 µg, 100 µg, 250 µg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E Coli |
| Tag/Conjugation | This protein contains an N-terminal tag and m |
| Purity | >95% as determined by SDS-PAGE and HPLC. |
| Bioactivity | Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 +/- 0.6 mM |
| Endotoxin | Low endotoxin available upon request. |
| SwissProt/Accession | P23368 |
| Gene | ME2 |
| Sequence | MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG RNTLIQFEDF GNHNAFRFLR KYREKYCTFN DDIQGTAAVA LAGLLAAQKV ISKPISEHKI LFLGAGEAAL GIANLIVMSM VENGLSEQEA QKKIWMFDKY GLLVKGRKAK IDSYQEPFTH SAPESIPDTF EDAVNILKPS TIIGVAGAGR LFTPDVIRAM ASINERPVIF ALSNPTAQAE CTAEEAYTLT EGRCLFASGS PFGPVKLTDG RVFTPGQGNN VYIFPGVALA VILCNTRHIS DSVFLEAAKA LTSQLTDEEL AQGRLYPPLA NIQEVSINIA IKVTEYLYAN KMAFRYPEPE DKAKYVKERT WRSEYDSLLP DVYEWPESAS SPPVITE+HH HHHH |
| Description | Recombinant Human NAD-dependent malic enzyme, mitochondrial protein (ME2) (Active) |
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