Bioactivity |
ab134433 is shown to be non-reactive for HbsAG, antiHCV, anti-HBc, and negative for anti-HIV1 2 by FDA approved tests. Activity of ab134433 was assayed employing the synthetic chromogenic peptide Methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide (0.3 mM final concentration) in 0.1 M Tris-HCl, pH 8 at 25°C. One unit will release 1 µmole of 4-nitroanilide per minute from the substrate at 25°C, pH 8.0. Under the specified conditions the specific activity is >18 U/mg. ab134433 is approximately 90% active by titration with &alhpa;1- antitrypsin.
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