| Name |
Active E. coli surA full length protein
|
| Supplier |
Abcam
|
| Catalog |
ab99241
|
| Category |
Protein
|
| Prices |
$239.00, $883.00
|
| Sizes |
100 µg, 500 µg
|
| Applications |
SDS-PAGE MS FA
|
| Species Reactivities |
Escherichia coli
|
| Nature |
Recombinant
|
| Source |
E. coli
|
| Tag/Conjugation |
His tag N-Terminus
|
| Purity |
> 95 % by SDS-PAGE. ab99241 is purified using conventional chromatography techniques.
|
| Bioactivity |
Specific activity is > 450 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. Add 10 µl of recombinant surA protein with 1 µg in assay buffer. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) Record the increase in A405 nm for 30 minutes at 25°C.
|
| SwissProt/Accession |
P0ABZ6
|
| Gene |
surA
|
| Residue |
21 to 428
|
| Sequence |
MGSSHHHHHH SSGLVPRGSH M APQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN
|
| Supplier Page |
Shop
|
15% SDS-PAGE showing ab99241 at approximately 47.3kDa (3µg).