Active human PDE1C full length protein
| Name | Active human PDE1C full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198450 |
| Category | Protein |
| Prices | $1,250.00 |
| Sizes | 10 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | >= 80 % by SDS-PAGE. |
| Bioactivity | Specific Activity: ≥ 15,000 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C. Assayed using a PDE1C Assay Kit. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl 2 , 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198450 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. |
| SwissProt/Accession | Q14123 |
| Gene | PDE1C |
| Residue | 2 to 634 |
| Sequence | ESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQL ERGEASVVDLKKNLEYAATVLESVYIDETRRLLDTEDELSDIQSDAVPSE VRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFVERMYRRTSNMV GLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISR FKIPISALVSFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVAN WLTELEIFAIIFSAAIHDYEHTGTTNNFHIQTRSDPAILYNDRSVLENHH LSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIKA MKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQ GDREAELGLPFSPLCDRKSTMVAQSQVGFIDFIVEPTFTVLTDMTEKIVS PLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEGSAPINNSVISV DYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEME AKSQAEEGASGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQ QQNGDFKDGKNKTDKKDHSNIGNDSKKTDDSQE |
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