Active human Peroxiredoxin 2 full length protein
| Name | Active human Peroxiredoxin 2 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab79947 |
| Category | Protein |
| Prices | $897.00 |
| Sizes | 100 µg |
| Applications | FA SDS-PAGE WB |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | Specific Activity: 149 pmol/min/µg. 3.45 µg of Peroxiredoxin 2 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H 2 O 2 . NADPH oxidation was monitored (fluorescence decrease) for the next 10 min. |
| SwissProt/Accession | P32119 |
| Gene | PRDX2 |
| Sequence | Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain. |
| Supplier Page | Shop |
Product images
Lane 1: ab79947 on 14% SDS-PAGE, Coomassie staining, 10µg. Lane 2: Protein marker.
Anti-Peroxiredoxin 2 antibody ( ab86295 ) at 1 µg/ml + Active human Peroxiredoxin 2 full length protein (ab79947) at 0.01 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080 ) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Exposure time : 2 minutes