Active human PGP9.5 full length protein
| Name | Active human PGP9.5 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab53962 |
| Category | Protein |
| Prices | $192.00 |
| Sizes | 25 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | Specific activity: > 300 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C. Activity Assay: Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) Add 50ul of 1uM Ubiquitin-AMC. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - Ubiquitin-AMC - 96 Well Polystyrene Microplate, black - Fluorescent plate reader (PerkinElmer, VICTOR X3) |
| SwissProt/Accession | P09936 |
| Gene | UCHL1 |
| Residue | 1 to 223 |
| Sequence | MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA |
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Product images
SDS Page analysis of ab53962 (lane 2) run on a 15% SDS-PAGE gel with molecular weight markers (lane 1).